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Rapidly emerging at the intersection of nanotechnology, materials science, and molecular biology, the field of nanobiotechnology promises to elucidate many life processes at the molecular-level previously invisible to human inquiry, and thereby dramatically to transform diagnostics, therapy, and drug discovery in this postgenomic world. In Protein Nanotechnology: Protocols, Instrumentation, and Applications, leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Protein Nanotechnology: Protocols, Instrumentation, and Applications provides investigators a wide variety of readily reproducible techniques and methods for exploring both protein functions and structures at the nanoscale level and their powerful new biological and medical applications
Key Features:
Cutting-edge methods for studying protein functions and structure at the nanoscale level
Discussion of nanostructured systems, nanomaterials, and optical nanosensors
Novel biological and medical applications
First book devoted exclusively to protein nanotechnology
Step-by-step instructions to ensure successful results
Tricks of the trade and notes on troubleshooting and avoiding known pitfalls
Table of Contents
Protein Nanotechnology: The New Frontier in Biosciences
Kinetics and Mechanisms of Protein Crystallization at the Molecular Level
Nanostructured Systems for Biological Materials
Nanomaterials of Drug Delivery Systems for Tissue Regeneration
Nanotechnology with S-Layer Proteins
Folding of Beta-Structured Fibrous Proteins and Self-Assembling Peptides
Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors
Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy
Carbon Nanotubes and Nanowires for Biological Sensing
Carbon Nanotube Systems to Communicate with Enzymes
Molecularly imprinted Polymers for Biomolecular Recognition
Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis
Bacterial Virus 29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology
Construction of Ordered Protein Arrays
Bioengineering and Characterization of DNA-Protein Assemblies Floating on Supported Membranes
Nanosystems for Biosensing: Multianalyte Immunoassay on a Protein Chip
Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells
Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity
Protein Amyloidose Misfolding: Mechanisms, Detection, and Pathological Implications
Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution
ISBN - 9788184893984
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Pages : 480
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