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  Gene Probes Vol. 1& 2
 

Gene Probes Vol. 1& 2

by Hames B. D.

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  Contents : vol. I : Introduction * Strategies for using gene probes * Preparati on of nucleic acids for use as probes * DNA rafiolabelling and detection * Non-radioactive labelling of DNA probes * Preparation synthetic oligodeoxynucleotide probes * Probe preparation by the polymerase chain reaction * Synthesis of riboprobes * Antobody probes. * Contents : vol. 2 : Hybridization strategy * Screening recombinant DNA libraries by hybridization and amplication * Screening human recombinant libraries with nucleic acid probes * Anotibody screening of bacterial expression systems * Isolation of DNA clones by direct exoression in mammalian cells * Genomic mapping and gene structure analysis * Analysis of gene expression by blotting techniques * In situ hybridization of nucleic acid probes to celular RNA* Application of chromosomal in sity hybridizatio * Analysis of gene expression anaysis hy PCR : a quantitative aproach * Analysis of protein expression by immunocytochemistry * Gene trageting in mammalian cells * Dricted mutaenesis and mutant anaslysis * Human gene mutational analysis 1995 P.B.
 


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