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An extensive compilation of reviews, opinions, and applications by today`s top researchers and professionals in the field, Principles of Mass Spectrometry Applied to Biomolecules presents the latest information on the basic principles of mass spectrometry as they relate to the life sciences, with special focus on the inherent challenges posed by varying degrees of biomolecular size and flexibility.
Featuring the professional contributions of more than twenty-five experts from academia and industry, the text helps readers to understand how to use recent advances while recognizing and discussing challenges associated with analysis of biomolecules using mass spectrometry.
Topics have been arranged into three distinct sections—Structures and Dynamics of Gas-Phase Biomolecules; Activation, Dissociation, and Reactivity; and Thermochemistry and Energetics—and
Include:
Spectroscopy
Energetics and mechanisms of peptide fragmentation
Electron capture dissociation
Ion-ion and ion-molecule reactions
Reaction dynamics
Collisional activation
Soft-landing
Protein structure and interactions
Thermochemistry
This important work provides today`s scientists and researchers with an in-depth understanding of the fundamental principles underlying the existing analytical approaches in this exciting and growing field, complete with its successes and limitations, so that they can develop new and innovative ways of using these techniques in their own work.
Contents
Contributors.
Preface.
STRUCTURES AND DYNAMICS OF GAS-PHASE BIOMOLECULES.
1. Spectroscopy of neutral Peptides in the Gas Phase-Structure, Reactivity, Microsolvation, Molecular Recognition
2. Probing the Electronic Structure of Fe-S Clusters: Ubiquitous Electron Transfer Centers in Metalloproteins Using Anoin Photoelectron Spectroscopy in the Gas Phase
3. Ion/Molecule Reactions and H/D Exchange for Structural Characterization of Biomolecules
4. Understanding Protein Interactions and their Representation in the Gas Phase of the Mass Spectrometer
5. Protein Structure and Folding in the Gas Phase: Ubiquitin and Cytochrome c
6. Dyunamical Simulations of Photoionization of Small Biological Molecules
7. Intramolecular Vibrational Energy Redistribution and Ergodicity of Biomolecular Dissociation
PART II: ACTIVATION, DISSOCIATION AND REACTIVITY.
8. Peptide Fragmentation Overview
9. Peptide Radical Cations
10. Photodissociation of Biomolecule Ions: Progress, Possibilities, and Perspectives Coming From Small-Ion Models
11. Chemical Dynamics Simulations of Energy Transfer and Unimolecular Decomposition in Collision-Induced Dissociation (CID) and Surface-Induced Dissociation (SID)
12. Ion Soft Landing: Instrumentation, Phenomena and Applications
13. Electron Capture Dissociation and Other Ion-electron Fragmentation Reactions
14. Biomolecule Ion/Ion Reactions
PART III: THERMOCHEMISTRY AND ENERGETICS.
15. Thermochemistry Studies of Biomolecules
16. Energy and Entropy Effects in the Gas Phase Dissociation of Peptides and Proteins
Index.
JULIA LASKIN, PHD, is a Senior Research Scientist in the Fundamental Sciences Division at the Pacific Northwest National Laboratory in Richland, Washington.
The late CHAVA LIFSHITZ, PHD, was an Archie and Marjorie Sherman Professor of Chemistry in the Department of Physical Chemistry at The Hebrew University in Jerusalem, Israel. She was also on the editorial boards of Mass Spectrometry Reviews and the International Journal of Mass Spectrometry.ISBN - 9788126546350
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Pages : 706
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