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Protein Purification: Theory and Techniques

	Protein Purification: Theory And Techniques by K S Dayanada
	Price : Rs 495.00
	Your Price : Rs 445.50

Description: Protein Purification – Theory and Techniques provides a good reference for the techniques and strategies involved in the purification of antibodies and recombinant proteins. The detailed practical guidelines given in the book aim particularly at laboratory scale purification. The book is designed to give the laboratory worker the information needed to undertake the challenge of designing a strategy to produce a purified protein from a crude mixture or cell debris.

The book also reflects personal preferences amongst the multitude of methods that may be used for protein fractionation. There is no single correct way to solve the problem, and probably teams could choose several alternative procedures and come up with a satisfactory solution. The approach and the results are discussed and compared in this book.

Contents: PROTIEN PURIFICATION: Protein Purification • Preparation • Three Phase Purification Strategy • Capture • Intermediate Purification • Polishing • Examples of Protein Purification Strategies • Sample Stroage Conditions • Principles and Standard Conditions for Purification Technique • Antibody Purification: Antibody Purification • Sample Preparation • Sample, Rapid Purification by Affinity Chromatography • Immunoprecipitation • Multi-Step Purification Strategies • Removal of Specific Contaminants after Initial Purification • Large-Scale Purification • RECOMBINANT PROTEINS:
• Purification of Recombinant Protein • GST Fusion Protein • (His)6 Fusion Proteins • Handling Inclusion Bodies • Harvesting and Extraction of Recombinant Proteins • Buffer Exchange and Desalting of Recombinant Proteins • Simple Purification of other Recombinant Proteins • Multi-Step Purification of Recombinant ProteinsISBN - 9788130900384

Pages : 234