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Description: The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language. The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.
Contents: 1.Principle, working and uses of instruments: Autoclave.. 2.Preparation of culture media 3. Preparation of Standard Solution 4. Isolation of bacteria by streak plate method 5. Isolation of bacteria by pour plate method 6. To determine the growth characteristics of E.Coli 7. Growth charatersitics of E.coli using turbidimetric method 8. Isolation of plasmid from E.coli 9. Isolation of bacterial genomic DNA from E.coli 10. To determine the DNA molecular size 11. Purification of DNA 12. To perform Bacteriophage titration 13. To perform restriction digestion of DNA with enzyme 14.To perform tranformation of the given bacterial population 15. DNA amplification by Polymerase chain reaction 16. Preparation of Plant Tissue culture media 17. To isolate protoplast from the plant tissue 18. Vialibilty testing of protoplast 19. Demonstrate of Protoplast fusion using polyethylene glycol 20. Demonstration of Callus culture techniques 21. Initiation of callus from radical tip of Phaseolus Vulgaris 22.Demonstration of Cell Suspension Culture 23. Demonstration of Androgensis is Datura 24. Demonstration os Somatic embryogenesis 25. Organogensis 26.Growth Index 27.Artificial Seeds 28. Transfer of Planets 29. Isolation of Genomic DNA 30.Isolation of Agrobacteruim 31. Agro Bacterium 32. B-Gluronidase 33.DNA polymorphism 34. DNA polymorphism *viva Voce, Refernces, Index ISBN Description: The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language. The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.
Contents: 1.Principle, working and uses of instruments: Autoclave.. 2.Preparation of culture media 3. Preparation of Standard Solution 4. Isolation of bacteria by streak plate method 5. Isolation of bacteria by pour plate method 6. To determine the growth characteristics of E.Coli 7. Growth charatersitics of E.coli using turbidimetric method 8. Isolation of plasmid from E.coli 9. Isolation of bacterial genomic DNA from E.coli 10. To determine the DNA molecular size 11. Purification of DNA 12. To perform Bacteriophage titration 13. To perform restriction digestion of DNA with enzyme 14.To perform tranformation of the given bacterial population 15. DNA amplification by Polymerase chain reaction 16. Preparation of Plant Tissue culture media 17. To isolate protoplast from the plant tissue 18. Vialibilty testing of protoplast 19. Demonstrate of Protoplast fusion using polyethylene glycol 20. Demonstration of Callus culture techniques 21. Initiation of callus from radical tip of Phaseolus Vulgaris 22.Demonstration of Cell Suspension Culture 23. Demonstration of Androgensis is Datura 24. Demonstration os Somatic embryogenesis 25. Organogensis 26.Growth Index 27.Artificial Seeds 28. Transfer of Planets 29. Isolation of Genomic DNA 30.Isolation of Agrobacteruim 31. Agro Bacterium 32. B-Gluronidase 33.DNA polymorphism 34. DNA polymorphism *viva Voce, Refernces, Index
ISBN Description: The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language. The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.
Contents: 1.Principle, working and uses of instruments: Autoclave.. 2.Preparation of culture media 3. Preparation of Standard Solution 4. Isolation of bacteria by streak plate method 5. Isolation of bacteria by pour plate method 6. To determine the growth characteristics of E.Coli 7. Growth charatersitics of E.coli using turbidimetric method 8. Isolation of plasmid from E.coli 9. Isolation of bacterial genomic DNA from E.coli 10. To determine the DNA molecular size 11. Purification of DNA 12. To perform Bacteriophage titration 13. To perform restriction digestion of DNA with enzyme 14.To perform tranformation of the given bacterial population 15. DNA amplification by Polymerase chain reaction 16. Preparation of Plant Tissue culture media 17. To isolate protoplast from the plant tissue 18. Vialibilty testing of protoplast 19. Demonstrate of Protoplast fusion using polyethylene glycol 20. Demonstration of Callus culture techniques 21. Initiation of callus from radical tip of Phaseolus Vulgaris 22.Demonstration of Cell Suspension Culture 23. Demonstration of Androgensis is Datura 24. Demonstration os Somatic embryogenesis 25. Organogensis 26.Growth Index 27.Artificial Seeds 28. Transfer of Planets 29. Isolation of Genomic DNA 30.Isolation of Agrobacteruim 31. Agro Bacterium 32. B-Gluronidase 33.DNA polymorphism 34. DNA polymorphism *viva Voce, Refernces, Index
ISBN 8121932009
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